Investigation and comparison of bench-scale anaerobic digestion system performance under thermophilic and mesophilic conditions for producing biomethane from solid organic wastes

Atrkar Roshan, Saina; Yousefi, Leila; Abdalisousan, Ashkan

doi:10.22059/ees.2023.2005533.1432

Investigation and comparison of bench-scale anaerobic digestion system performance under thermophilic and mesophilic conditions for producing biomethane from solid organic wastes

Document Type : Research Paper

Authors

¹ Department of Energy Engineering and Economic, Science and Research Branch, Islamic Azad University, Tehran, Iran.

² Department of Chemistry, Islamshahr Branch, Islamic Azad University, Islamshahr, Tehran, Iran.

³ Department of Engineering and Technology, Astara Branch, Islamic Azad University, Astara, Iran.

10.22059/ees.2023.2005533.1432

Abstract

The outcomes of every batch of anaerobic digesters vary due to differences in equipment, feed composition, and operating conditions. The performance of a bench-scale anaerobic digestion system was investigated in this study with the goal of biomethane production, considering mesophilic requirements at 37°C and thermophilic conditions at 57°C. Three experiments were carried out under mesophilic and thermophilic conditions. A blend of water and solid organic waste was used in the first experiment. The second test involved a mixture of water, material that had undergone prior digestion, and solid organic waste. The third trial incorporated water, previously digested material, solid organic waste, and cow manure as part of the feed. Based on the result, in the third experiment, over 32 days within mesophilic conditions, the average daily biogas and biomethane production per unit of feed mass was quantified as 1610 and 447.10 ml, respectively. Furthermore, the measurements indicated 62.22% and 99.99% increases compared to similar feed subjected to thermophilic conditions. Moreover, the data displayed growth rates of 8.05%, 96.04%, and a substantial rise of 69.47% and 147.7% compared to the outcomes of the second and third experiments performed under mesophilic conditions. Additionally, it can be included that the digestion period of the third test was 60 days shorter than that of the second and one day less than the first trial when operating under mesophilic conditions. As a result, the most effective method for anaerobic digestion involved blending solid organic waste, previously digested material, cow manure, and water under mesophilic conditions.

Keywords

References

[1] Aydram, R., Haj Agha Alizade, H., Rasouli, M., Shadidi, B., Simplex Centroid Mixture Design for Optimizing and Promoting the Anaerobic Co-digestion Performance of Sheep Blood and Cheese Whey, Journal of Renewable Energy and Environment (2021) 8(3): 8-15. https://doi.org/10.30501/jree.2021.251583.1151

[2] Sutaryo, S., Ward, A. J., Moller, HB., Ammonia inhibition in thermophilic anaerobic digestion of dairy cattle manure, Journal of the Indonesian Tropical Animal Agriculture (2014) 39(2):83-90. https://doi.org/10.14710/jitaa.39.2.83-90

[3] Ferrer, I., Vázquez, F., Font, X., Comparison of the Mesophilic and Thermophilic Anaerobic Sludge Digestion from an Energy Perspective, Journal of Residuals Science & Technology (2011) 8(2): 81-87.

[4] Riggio, S., Hernandéz-Shek, MA., Torrijos, M., Vives, G., Esposito, G, van Hullebusch, ED., Steyer, JP., Escudié, R., Comparison of the Mesophilic and Thermophilic Anaerobic Digestion of Spent Cow Bedding in Leach-bed Reactors, Bioresource Technology (2017) 234: 466-471. https://doi.org/10.1016/j.biortech.2017.02.056

[5] Moset, E., Poulsen, M., Wahid, R., Højberg, O., Møller, HB., Mesophilic Versus Thermophilic Anaerobic Digestion of Cattle Manure: Methane Productivity and Microbial Ecology, Microbial Biotechnology (2015) 8(5): 787–800. https://doi.org/10.1111/1751-7915.12271

[6] Guo, X., Kang, K., Shang, G., Yu, X., Qiu, L., Sun, G., Influence of Mesophilic and Thermophilic Conditions on the Anaerobic Digestion of Food Waste: Focus on the Microbial Activity and Removal of Long Chain Fatty Acids, Waste Management & Research (2018) 36(11):1106-1112. https://doi.org/10.1177%2F0734242X18801195

[7] Yirong, C., Banks, CJ., Heaven, S., Comparison of Mesophilic and Thermophilic Anaerobic Digestion of Food Waste, Faculty of Engineering and the Environment, University of Southampton, Southampton (2013), SO17 1BJ, UK.

[8] Yousefi, L., The Biogas Handbook (Science, Production and Application), Part ǁ: Plant Design, Engineering, Process Optimization and Digestate Utilization. (2021), Azad Academic Press, (in Farsi).

[9] Singh, B., Szamosi, Z., Siménfalvi, Z., State of the Art on Mixing in an Anaerobic Digester: A Review, Renewable Energy (2019) 141: 922-936. https://doi.org/10.1016/j.renene.2019.04.072.

[10] Anonymous., Compost – Physical and Chemical Specifications, Institute of Standards and Industrial Research of Iran (10716) (1991), 1st. edition, (in Farsi).

[11] Anonymous., Experimental Method for Determining Amount of Soil Moisture, Institute of Standards and Industrial Research of Iran (1677) (1991), 3st. edition, (in Farsi).

[12] Anonymous., Compost – Sampling and Physical and Chemical Tests Method, Institute of Standards and Industrial Research of Iran (13320) (1991), 1st. edition, (in Farsi).

[13] Yousefi, L., The Biogas Handbook (Science, Production and Application), Part 1: Biomass Resources, Feed Processing and Biogas Production. (2019), Atran Publications, (in Farsi).

[14] Palatsi, J., Laureni, M., Andrés, MV., Flotats, X., Nielsen, HB., Angelidaki, I., Strategies for Recovering Inhibition Caused by Long Chain Fatty Acids on Anaerobic Thermophilic Biogas Reactors, Bioresource Technology (2009) 100 (20): 4588 - 4596. https://doi.org/10.1016/j.biortech.2009.04.046)

[15] Nosrati, M., Shojaosadati, S., Sreekrishnan, T., Mukhopadhyay, S., Inhibition of Thermophilic Anaerobic Digestion of Waste Food by Long Chain Fatty Acids and Propionate, Iranian Journal of Biotechnology (2004) 2(4): 261-268. https://doi.org/10.1139/A10-011

[16] Palatsi, J., Illa, J., Prenafeta-Boldú, FX., Laureni, M., Fernandez, B., Angelidaki, I., Flotats, X., Long-chain Fatty Acids Inhibition and Adaptation Process in Anaerobic Thermophilic Digestion: Batch Tests, Microbial Community Structure and Mathematical Modelling, Bioresource Technology (2010) 101(7): 2243-2251. https://doi.org/10.1016/j.biortech.2009.11.069

[17] Yousefi, L, Effect of Mixing Digested with Municipal Solid Organic Waste in Biogas Production Through Anaerobic Digestion Bath System under Mesophilic Conditions, Journal of Green Chemistry and Sustainable Technologies (2020) 3: 61-69. http://gcst.ccerci.ac.ir/article_112889_b901878e9f9e3fe8b61ac6f2652f582f.pdf) (in Farsi).

[18] Zhang, W., Chen, B., Li, A., Zhang, L., Li, R., Yang, T., Xing, W., Mechanism of Process Imbalance of Long-term Anaerobic Digestion of Food Waste and Role of Trace Elements in Maintaining Anaerobic Process Stability, Bioresource Technology (2018) 275:172-182. https://doi.org/10.1016/j.biortech.2018.12.052

[19] Ma, J., Zhao, QB., Laurens, LLM., Jarvis, EE., Nagle, NJ., Chen, S., Frear, CS., Mechanism, Kinetics and Microbiology of Inhibition Caused by Long-chain Fatty Acids in Anaerobic Digestion of Algal Biomass, Biotechnol Biofuels, (2015) 8: 141. https://doi.org/10.1186/s13068-015-0322-z

[20] Yenigün, O., Burak Demirel, B., Ammonia Inhibition in Anaerobic Digestion: A Review, Process Biochemistry (2013) 48(5–6): 901-911. https://doi.org/10.1016/j.procbio.2013.04.012

[21] Shihwu Sung, S., Liu T., Ammonia Inhibition on Thermophilic Anaerobic Digestion, Chemosphere (2003) 53(1): 43-52. https://doi.org/10.1016/S0045-6535(03)00434-X

[22] Benabdallah El Hadj, T., Astals, S., Galí, A., Mace, S., Mata-Alvarez, J., Ammonia Influence in Anaerobic Digestion of OFMSW, Water Science Technology (2009) 59(6):1153-8. https://doi.org/10.2166/wst.2009.100

[23] Capson-Tojo, G., Moscoviz, R., Astals, S., Robles, Á., Steyer, JP., Unraveling the Literature Chaos Around Free Ammonia Inhibition in Anaerobic Digestion, Renewable and Sustainable Energy Reviews (2020) 117: 109487. https://doi.org/10.1016/j.rser.2019.109487

[24] Fischer, MA., Ulbricht, A., Neulinger, SC., Refai, S., Waßmann, K., Künzel, S., Schmitz, RA., Immediate Effects of Ammonia Shock on Transcription and Composition of a Biogas Reactor Microbiome, Frontiers in Microbiology Journal (2019) 10: 2064. https://doi.org/10.3389/fmicb.2019.02064

[25] Myszograj, S., Stadnik, A., Płuciennik-Koropczuk, E., The Influence of Trace Elements on Anaerobic Digestion Process, Civil and Environmental Engineering Reports Journal (2018) 28(4): 105-115. https://doi.org/10.2478/ceer-2018-0054